Single-molecule enzymology à la Michaelis-Menten
نویسندگان
چکیده
منابع مشابه
Single-molecule enzymology à la Michaelis-Menten.
Over the past 100 years, deterministic rate equations have been successfully used to infer enzyme-catalysed reaction mechanisms and to estimate rate constants from reaction kinetics experiments conducted in vitro. In recent years, sophisticated experimental techniques have been developed that begin to allow the measurement of enzyme-catalysed and other biopolymer-mediated reactions inside singl...
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This paper summarizes our present theoretical understanding of single-molecule kinetics associated with the Michaelis-Menten mechanism of enzymatic reactions. Single-molecule enzymatic turnover experiments typically measure the probability density f(t) of the stochastic waiting time t for individual turnovers. While f(t) can be reconciled with ensemble kinetics, it contains more information tha...
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Biological nanopores are a class of membrane proteins that open nanoscale water conduits in biological membranes. When they are reconstituted in artificial membranes and a bias voltage is applied across the membrane, the ionic current passing through individual nanopores can be used to monitor chemical reactions, to recognize individual molecules and, of most interest, to sequence DNA. In addit...
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Recent advances in single-molecule enzymology are reviewed. The theoretical underpinning of sincle-molecule enzymatic behaviors is discussed and exemplified by experiements and statistical analyses. In particular, the manifestations of the Michaelis-Menten mechanism, the kinetic scheme with sequential intermediates, and dynamic disorder in single molecule data are presented. A survey of current...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2014
ISSN: 1742-464X
DOI: 10.1111/febs.12663